Translational_Unit

Part:BBa_K4382011:Design

Designed by: UZAIR MOTIWALA   Group: iGEM22_IISER_Bhopal   (2022-10-11)


Xylanase Gene Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4128
    Illegal SpeI site found at 4099
    Illegal PstI site found at 200
    Illegal PstI site found at 3740
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4128
    Illegal NheI site found at 205
    Illegal SpeI site found at 4099
    Illegal PstI site found at 200
    Illegal PstI site found at 3740
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4128
    Illegal BglII site found at 3382
    Illegal BamHI site found at 929
    Illegal BamHI site found at 2179
    Illegal BamHI site found at 4164
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4128
    Illegal SpeI site found at 4099
    Illegal PstI site found at 200
    Illegal PstI site found at 3740
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4128
    Illegal SpeI site found at 4099
    Illegal PstI site found at 200
    Illegal PstI site found at 3740
    Illegal NgoMIV site found at 4627
    Illegal NgoMIV site found at 5041
    Illegal AgeI site found at 4612
    Illegal AgeI site found at 5470
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 609
    Illegal BsaI.rc site found at 1215
    Illegal BsaI.rc site found at 2915
    Illegal SapI.rc site found at 663


Design Notes

The basic parts used are extracted from Bacillus Subtilis genome.


Source

-NA-

References